The use of vinylcyclohexene diepoxide to create diminish ovarian reserve model on rats

Background: Infertility remains a significant issue in the world of health. Now Assisted Reproductive Technology (ART) is widely used to help couples with infertility. In ART diminish ovarian reserve patients have very low success rate pregnancy. To Diminished Ovarian Reserve (DOR), research conducted using an animal model. vinylcychlohexene diepoxide (VCD) has ovotoxic effects. This study looked at use VCD inducing targeted DOR conditions animals (rats), create model that could be for future studies infertility. Methods: Adult female Rattus norvegicus were this study. All given injections 80 mg/kg intraperitoneally 15 days. An examination Follicle Stimulating Hormone (FSH) levels will carried out on days 0,3,5,7, and 15. mimic IVF process, all rats got injection 10 IU Pregnant Mare Serum Gonadotropin (PMSG) human Chorionic (hCG) confirm DOR. Ovarectomy was performed, dominant follicles taken, denudation out, oocytes cultured 12 hours. Then, assessed through microscope. Results: Starting from day 5 post induction, there increase FSH level group induced administered dose compared control experienced normal fluctuations. The concentration reached >50%. It found number degenerated had average 1.11. Oocytes Germinal-Vesicle (GV) stage 1.89, while Metaphase I (M1) 2.882. Lastly, (MII) which fully matured 1.117. Conclusions: VCD can become important tool needs